Proinsulin endopeptidase substrate specificities defined by site-directed mutagenesis of proinsulin
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چکیده
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Creating lipoxygenases with new positional specificities by site-directed mutagenesis.
In order to analyse the amino acid determinants which alter the positional specificity of plant lipoxygenases (LOXs), multiple LOX sequence alignments and structural modelling of the enzyme-substrate interactions were carried out. These alignments suggested three amino acid residues as the primary determinants of positional specificity. Here we show the generation of two plant LOXs with new pos...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)51468-x